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Body weight and hormone levels of male and female mice following GDX or sham surgeries. (A) Body weight for each male mouse was recorded on the day of surgery, at 3 weeks post-surgery, and continuing every 2 weeks thereafter until the endpoint. GDX mice gained less weight than sham-operated mice, and WT mice were heavier than AD mice. Group size: WT male Sham = 12, WT male GDX = 14; AD male Sham = 12, AD male GDX = 14. (B) Body weight for each female mouse was recorded with the same time course as male animals. All animals gained weight at the same rate following surgery. Group size: WT female Sham = 8, WT female GDX = 10; AD female Sham = 16, AD female GDX = 21. (C) Serum levels of testosterone in males at endpoint, measured by ELISA. GDX eliminated serum testosterone. WT male Sham = 11; WT male GDX = 12; AD male Sham = 12; AD male GDX = 13. (D) <t>Serum</t> <t>17β-estradiol</t> in females at endpoint, measured by radioimmunoassay. GDX significantly reduced serum estradiol. WT female Sham = 10, WT female GDX = 9; AD female Sham = 15; AD female GDX = 18. *q < 0.05, ***q < 0.001. Error bars represent SEM
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Body weight and hormone levels of male and female mice following GDX or sham surgeries. (A) Body weight for each male mouse was recorded on the day of surgery, at 3 weeks post-surgery, and continuing every 2 weeks thereafter until the endpoint. GDX mice gained less weight than sham-operated mice, and WT mice were heavier than AD mice. Group size: WT male Sham = 12, WT male GDX = 14; AD male Sham = 12, AD male GDX = 14. (B) Body weight for each female mouse was recorded with the same time course as male animals. All animals gained weight at the same rate following surgery. Group size: WT female Sham = 8, WT female GDX = 10; AD female Sham = 16, AD female GDX = 21. (C) Serum levels of testosterone in males at endpoint, measured by ELISA. GDX eliminated serum testosterone. WT male Sham = 11; WT male GDX = 12; AD male Sham = 12; AD male GDX = 13. (D) <t>Serum</t> <t>17β-estradiol</t> in females at endpoint, measured by radioimmunoassay. GDX significantly reduced serum estradiol. WT female Sham = 10, WT female GDX = 9; AD female Sham = 15; AD female GDX = 18. *q < 0.05, ***q < 0.001. Error bars represent SEM
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Body weight and hormone levels of male and female mice following GDX or sham surgeries. (A) Body weight for each male mouse was recorded on the day of surgery, at 3 weeks post-surgery, and continuing every 2 weeks thereafter until the endpoint. GDX mice gained less weight than sham-operated mice, and WT mice were heavier than AD mice. Group size: WT male Sham = 12, WT male GDX = 14; AD male Sham = 12, AD male GDX = 14. (B) Body weight for each female mouse was recorded with the same time course as male animals. All animals gained weight at the same rate following surgery. Group size: WT female Sham = 8, WT female GDX = 10; AD female Sham = 16, AD female GDX = 21. (C) Serum levels of testosterone in males at endpoint, measured by ELISA. GDX eliminated serum testosterone. WT male Sham = 11; WT male GDX = 12; AD male Sham = 12; AD male GDX = 13. (D) <t>Serum</t> <t>17β-estradiol</t> in females at endpoint, measured by radioimmunoassay. GDX significantly reduced serum estradiol. WT female Sham = 10, WT female GDX = 9; AD female Sham = 15; AD female GDX = 18. *q < 0.05, ***q < 0.001. Error bars represent SEM
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Body weight and hormone levels of male and female mice following GDX or sham surgeries. (A) Body weight for each male mouse was recorded on the day of surgery, at 3 weeks post-surgery, and continuing every 2 weeks thereafter until the endpoint. GDX mice gained less weight than sham-operated mice, and WT mice were heavier than AD mice. Group size: WT male Sham = 12, WT male GDX = 14; AD male Sham = 12, AD male GDX = 14. (B) Body weight for each female mouse was recorded with the same time course as male animals. All animals gained weight at the same rate following surgery. Group size: WT female Sham = 8, WT female GDX = 10; AD female Sham = 16, AD female GDX = 21. (C) Serum levels of testosterone in males at endpoint, measured by ELISA. GDX eliminated serum testosterone. WT male Sham = 11; WT male GDX = 12; AD male Sham = 12; AD male GDX = 13. (D) Serum 17β-estradiol in females at endpoint, measured by radioimmunoassay. GDX significantly reduced serum estradiol. WT female Sham = 10, WT female GDX = 9; AD female Sham = 15; AD female GDX = 18. *q < 0.05, ***q < 0.001. Error bars represent SEM

Journal: Biology of Sex Differences

Article Title: Gonadal hormones contribute to sex differences in behavior, pathology and epigenetic modifications in the 3×Tg-AD mouse model of Alzheimer’s disease

doi: 10.1186/s13293-025-00790-9

Figure Lengend Snippet: Body weight and hormone levels of male and female mice following GDX or sham surgeries. (A) Body weight for each male mouse was recorded on the day of surgery, at 3 weeks post-surgery, and continuing every 2 weeks thereafter until the endpoint. GDX mice gained less weight than sham-operated mice, and WT mice were heavier than AD mice. Group size: WT male Sham = 12, WT male GDX = 14; AD male Sham = 12, AD male GDX = 14. (B) Body weight for each female mouse was recorded with the same time course as male animals. All animals gained weight at the same rate following surgery. Group size: WT female Sham = 8, WT female GDX = 10; AD female Sham = 16, AD female GDX = 21. (C) Serum levels of testosterone in males at endpoint, measured by ELISA. GDX eliminated serum testosterone. WT male Sham = 11; WT male GDX = 12; AD male Sham = 12; AD male GDX = 13. (D) Serum 17β-estradiol in females at endpoint, measured by radioimmunoassay. GDX significantly reduced serum estradiol. WT female Sham = 10, WT female GDX = 9; AD female Sham = 15; AD female GDX = 18. *q < 0.05, ***q < 0.001. Error bars represent SEM

Article Snippet: Estradiol levels in serum of females was assayed using the Double Antibody RIA 17β-Estradiol kit (MP Biomedicals, Irvine, CA USA).

Techniques: Enzyme-linked Immunosorbent Assay, RIA Assay